Abstract: Water, the primary volatile constituent in anatectic melt and terrestrial magma, has a significant effect on the physical and chemical properties of the melt. Due to limitations in analytical techniques and the fugitive nature of anatectic melts, it is extremely difficult to quantitatively determine water concentration and water speciation, resulting in a very limited understanding of the formation mechanism and evolution process for partial melting in subduction zone. Confocal micro-Raman spectroscopy, which has the advantages of high spatial resolution, fast, nondestructive analysis and simple sample preparation, severs the purpose of detecting small melt inclusions. Additionally, based on the principle that there is a good linear relationship between the height/intensity of Raman spectrum peak and the content of corresponding groups, the internal calibration and external calibration are established for the quantitative analysis of water content and water speciation in silicate melt inclusions with synthesized silicate glass as the standard sample. As a newly developed technique and method, more and more geologists pay attention to it. However, a large number of researches are still focused on the deduction and correction of the analytical method itself, while the research on natural samples is relatively lacking. Limited research indicates that the method can be widely used in magmatites and high-grade metamorphic rocks. It achieves quantitatively determining the water content of matrix or melt inclusion in porphyry, thereby effectively tracing the rheological behavior of magma during magma intrusion or eruption. Water content and speciation of the anatectic melt in the continental subduction zone provides tight constraints for continental crust differentiation and melt metasomatites at the slab-wedge interface.