Effect and Mechanism of Fe(II) Oxygenation on Activities of Iron and Manganese Cycling Functional Microbes
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摘要: 地质微生物是沉积环境中铁、锰氧化还原循环的主要驱动因子，铁锰共存环境中二价铁氧化对不同铁、锰循环功能微生物活性的影响差异和机制尚不清楚.以铁还原菌Shewanella oneidensis MR-1、铁氧化菌Pseudogulbenkiania sp.strain 2002、锰氧化菌Pseudomonas putida MnB1和Leptothrix discophora SS-1作为代表性的铁、锰循环功能微生物，利用平板计数、荧光显微镜等手段探究了Fe(II)氧化对功能微生物活性的影响差异及机制.结果表明0.05 mM Fe2+氧化60 min可使MR-1和MnB1的活菌数量降低4~5个数量级，SS-1及S.2002无显著失活.Fe(II)氧化产生的吸附态。OH和胞内。OH是细菌失活的主要原因，胞外H2O2、胞外游离态。OH和三价铁氧化物是细菌失活的次要原因，SS-1及S.2002产生了氧化应激反应，成功抵御了活性氧化物种.Abstract: Geologic microorganisms are the main factors driving the redox cycling of iron and manganese in sedimentary environment. The effect and mechanism of Fe(II) oxygenation on the activities of different iron and manganese cycling functional microbes remain unexplored. In this study,it used Shewanella oneidensis MR-1,Pseudogulbenkiania sp. strain 2002,Pseudomonas putida MnB1,Leptothrix discophora SS-1 as the representative iron and manganese cycling functional microbes,it investigated the difference in effect and mechanisms of Fe(II) oxygenation on the activities of these four bacteria. Results show that the living cell counts of MR-1 and MnB1 decreased by 4-5 lg upon 60 min oxygenation of 0.05 mM Fe(II),but no significant inactivation of SS-1 and S.2002 was observed. The surface absorbed •OH and intracellular •OH produced from Fe(II) oxygenation were the dominant reasons for bacteria inactivation,the extracellular H2O2,extracellular dissolved •OH and Fe(III) oxides are minor causes for bacteria inactivation. SS-1 and S.2002 show oxidative stress responses during Fe2+ oxygenation,which helped to resist the damage of reactive oxygen species.
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Key words:
- Fe2+ /
- iron and manganese cycling /
- geologic microorganism /
- redox fluctuating /
- biogeochemistry
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图 1 Fe(II)氧化对Pseudogulbenkiania sp. strain 2002、Shewanella oneidensis MR-1、Pseudomonas putida MnB1和Leptothrix discophora SS1活性的影响
Fig. 1. Effects of Fe(II) oxygenation on the activities of Pseudogulbenkiania sp. strain 2002, Shewanella oneidensis MR-1, Pseudomonas putida MnB1 and Leptothrix discophora SS1
图 2 不同氧分压下Fe(II)氧化对Pseudomonas putida MnB1细菌活性的影响
Fig. 2. Effect of Fe(II) oxygenation on the activities of Pseudomonas putida MnB1 under various oxygen partial pressure
图 3 Fe(II)氧化影响前后细菌的荧光显微镜照片
Fig. 3. Florescence microscope photos of bacteria before and after Fe(II) oxygenation
图 4 Fe2+的氧化动力学(a、b)和H2O2、•OH累计浓度(c、d)
Fig. 4. The oxidation kinetics of Fe2+ (a, b) and the accumulative concentrations of H2O2 and •OH (c, d)
图 6 H2O2影响前后细菌的荧光显微镜照片
Fig. 6. Florescence microscope photos of bacteria before and after the reaction with H2O2
图 8 加入活性氧淬灭剂对细菌活性变化的影响
Fig. 8. Effect of •OH quencher on the variations of bacteria activities
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