Cultivation and Characters of Aerobic Granule Sludge on Pharmaceutical Wastewater in Continuous Flow Condition
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摘要: 在上流式好氧颗粒污泥床反应器中, 以厌氧颗粒污泥和好氧絮状活性污泥为接种泥, 采用人工配制的模拟废水, 成功培养出性能优异的好氧颗粒污泥.反应器内污泥浓度稳定在5g/L左右, 颗粒污泥粒径为0.5~2.0mm, 当进水COD为2000mg/L, 容积负荷为4.8kg/(m3·d)时, 系统对COD的去除率稳定在96%以上.通过扫描电镜观察, 好氧颗粒污泥是层状结构, 表面有大量丝状菌缠绕, 内部有短杆菌和空穴存在.逐步提高制药废水在进水中的比例, 经过47d的培养, 生物制药废水完全取代模拟废水, 系统对COD、NH3-N、TP的去除率分别稳定在90%、90%和70%以上.
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关键词:
- 连续流 /
- 好氧颗粒污泥 /
- 上流式好氧颗粒污泥床 /
- 制药废水 /
- 环境工程
Abstract: The successful cultivation of aerobic granular sludge with outstanding performance in airlift aerobic granular sludge bed reactor (AGSB) which was inoculated anaerobic granular sludge and aerobic flocculent sludge was performed using a synthetic wastewater mainly consisted of glucose as carbon source. The diameters of the granules ware 0.5-2.0mm and the sludge concentration in the reactor was in 5g/L or so. As the influent COD of 2000mg/L and the COD loading of 4.8kg/(m3·d) were employed, COD removal synthetic maintained more than 96%. Through the scanning electron microscopy observation, aerobic granular sludge was layered structure with a large number of filamentous bacteria winding on the surface, and some short bacillus and holes existing inside. After 47 days of cultivation, when the pharmaceutical wastewater completely replaced the synthetic water with gradually the increased pharmaceutical wastewater in the ratio of water, the COD, NH3-N, TP removal synthetic were more than 90%, 90%, and 70% respectively. -
图 1 实验装置示意
1.鼓风机；2.LZJ-10气体流量计进水控制阀；3.进水控制阀；4.进液控制阀；5.LZB-3液体流量计；6.兰格YZⅡ型蠕动泵；7.进液槽；8.潜水搅拌泵; 9.穿孔布水管；10.微孔曝气头；11.下集气罩；12.上集气罩；13.出气口；14.出水口；15.取样孔
Fig. 1. Schematic representation of the bioreactor
图 2 不同培养时间污泥形态
a.培养第5d；b.培养第15d；c.培养第25d；d.培养第35d
Fig. 2. The images of aerobic granules in differ incubation times
图 3 反应器内MLSS和SVI变化趋势
Fig. 3. The change of sludge and sludge volume index in the reactor
表 1 模拟废水成分
Table 1. Component of synthetic wastewater
基质名称 浓度(mg/L) 基质名称 浓度(mg/L) MgSO4·7H2O 100 NaHCO3 360~1080 CaCl2 40 蛋白胨 150 EDTA 30 牛肉膏 100 Na2MO4·2H20 0.065 NiCl2·6H2O 0.025 ZnSO4·7H20 0.12 KI 0.030 CoCl2·6H2O 0.21 MnCl2·4H20 0.120 H3B04 0.15 FeSO4·7H2O 2 CuSO4·5H2O 0.03 
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表 2 试验过程中进水水质特征
Table 2. Composition of inflow water
进水 水质特征(mg/L) COD BOD 氨氮 TN TP pH 制药废水 900~1100 400~500 40~50 50~60 1.5~2.0 7.5~8.5
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表 3 分析项目及其测试方法
Table 3. Analytical items and methods
序号 分析项目 测定方法 所用仪器 1 COD 连华科技COD快测 COD快测仪 2 NH4+-N 纳氏试剂分光光度法 分光光度计 3 TN 过硫酸钾氧化分光光度法 同上 4 TP 连华科技TP快测 TP快测仪 5 MLSS 标准重量法 干燥箱 6 DO 采用便携式溶解氧测定仪测定 便携测定仪 7 pH 玻璃电极法 酸度计 8 沉淀速度 选出几个粒径不同的颗粒污泥放在1L量筒中沉降，测其沉淀时间，沉速=水深/沉淀时间 1000mL量筒 9 污泥微观结构 扫描电镜 S-3400NⅡ
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[1] Chen, X.S., Xia, S.Q., Liu, G.C., 2007. Study on Physicochemical Characteristics of Aerobic Granular Sludge Cultivated in SBR. China Water & Wastewater, 23(9): 99-102 (in Chinese with English abstract). http://en.cnki.com.cn/Article_en/CJFDTOTAL-GSPS200709025.htm [2] Deng, F., Zhang, W., Xu, X., 2009. Hydraulic Characteristics and Influence Factors about Short-cut Nitrification of Continuous Aerobic Granule Reactor. Journal of PLA University of Science and Technology (Natural Science Edition), 10(6): 639-643 (in Chinese with English abstract). http://www.researchgate.net/publication/298184441_Hydraulic_characteristics_and_influence_factors_about_short-cut_nitrification_of_continuous_aerobic_granule_reactor [3] Hao, X.D., Chen, X.H., Dai, J., et al., 2006. Aerobic Granular Sludge Technology with Great Potential in Engineering Application. China Water & Wastewater, 22(8): 1-7 (in Chinese with English abstract). http://www.cnki.com.cn/Article/CJFDTotal-GSPS200608001.htm [4] Jin, A.F., Li, G, H., Zhang, X., 2012. The Risk Source Identification and Classification Methodology of Groundwater Pollution. Earth Science—Journal of China University of Geosciences, 37(2): 247-252 (in Chinese with English abstract). [5] Li, L.L., Zheng, X.L., Wu, J.W., et al. 2006. Effect of Nitrite on Aerobic Phosphate Uptake by Phosphate Accumulating Organisms. Environmental Science, 27(8): 1574-1579 (in Chinese with English abstract). http://www.sciencedirect.com/science/article/pii/S0043135404003070 [6] Liu, Y.Q., Tay, J.H., Moy, B.Y.P., 2006. Characteristics of Aerobic Granular Sludge in a Sequencing Batch Reactor with Variable Aeration. Appl. Microbiol Biotechnol, 71(5): 761-766. doi: 10.1007/soo253-005-0209-1 [7] Lu, C.R., Zhang, X.J., Zhang, Y., et al., 2001. Aerobic Sludge Granulation and Biological Phosphorus in Different Operating Conditions of SBR. Environmental Science, 22(2): 87-90(in Chinese with English abstract). http://en.cnki.com.cn/Article_en/CJFDTOTAL-HJXX200105013.htm [8] Ma, S.M., Zhong, Z.S., Wang, S.W., et al., 2012. Influencing Factors of Treatment of Wastewater Containing Benzotriazole with an Electro-Coagulation Method and the Optimization of Experimental Conditions. Earth Science—Journal of China University of Geosciences, 37(2): 337-344 (in Chinese with English abstract). http://www.researchgate.net/publication/291104585_Influencing_factors_of_treatment_of_wastewater_containing_benzotriazole_with_an_electro-coagulation_method_and_the_optimization_of_experimental_conditions [9] Morgenroth, E., Sherden, T., MCM, V.L., et al., 1997. Aerobic Granular Sludge in a Sequencing Batch Reactor. Water Reserch, 31(12): 3191-3194. doi: 10.1016/S0043-1354(97)00216-9 [10] Schwarzenbeck, N., Erley, R., Wllderer, P.A., 2004. Aerobic Granular Sludge in an SBR-system Treating Wastewater Rich in Particulate Matter. Water Sci. Technol. , 49(23): 41-46. http://europepmc.org/abstract/med/15303721 [11] State Environmental Protection Administration, 2002. The Water and Wastewater Monitoring Method (4th Edition). China Environmental Science Press, Beijing (in Chinese). [12] Zhou, D.D., Liu, M.Y., Hou, D.X., et al., 2012. Preliminary Approach on Formation Mechanism of Aerobic Granular Sludge During the Continuous Airlift Fluidized Bed Start-Up. Journal of Jilin University (Earth Science Edition), 42(1): 212-219 (in Chinese with English abstract). http://en.cnki.com.cn/Article_en/CJFDTOTAL-CCDZ201201029.htm [13] 陈雪松, 夏四清, 刘贵春, 2007. SBR好氧颗粒污泥的理化性质研究. 中国给水排水, 23(9): 99-102. doi: 10.3321/j.issn:1000-4602.2007.09.026 [14] 邓风, 张雯, 许欣, 等, 2009. 连续流好氧颗粒污泥反应器水力特性及亚硝化影响因素. 解放军理工大学学报(自然科学版), 10(6): 639-643. https://www.cnki.com.cn/Article/CJFDTOTAL-JFJL200906023.htm [15] 国家环境保护总局, 2002. 水和废水监测分析方法(第4版). 北京: 中国环境科学出版社. [16] 郝晓地, 陈新华, 戴吉, 等, 2006. 极具工程化潜力的好氧颗粒污泥技术. 中国给水排水, 22(8): 1-7. doi: 10.3321/j.issn:1000-4602.2006.08.001 [17] 金爱芳, 李广贺, 张旭, 2012. 地下水污染风险源识别与分级方法. 地球科学——中国地质大学学报, 37(2): 247-252. https://www.cnki.com.cn/Article/CJFDTOTAL-DQKX201202008.htm [18] 李玲玲, 郑西来, 吴俊文, 等, 2006. 亚硝酸盐对聚磷菌好氧摄磷的影响研究. 环境科学, 27(8): 1574-1579. doi: 10.3321/j.issn:0250-3301.2006.08.018 [19] 卢然超, 张晓健, 张悦, 等, 2001. SBR工艺运行条件对好氧污泥颗粒化和除磷效果的影响. 环境科学, 22(2): 87-90. doi: 10.3321/j.issn:0250-3301.2001.02.018 [20] 马生明, 钟佐燊, 王守伟, 等, 2012. 电凝聚法处理含苯并三唑废水的条件优化实验. 地球科学——中国地质大学学报, 37(2): 337-344. https://www.cnki.com.cn/Article/CJFDTOTAL-DQKX201202022.htm [21] 周丹丹, 刘孟媛, 侯典训, 等, 2012. 连续流气提式流化床启动过程中好氧颗粒污泥的形成机制. 吉林大学学报, 42(1): 212-219. https://www.cnki.com.cn/Article/CJFDTOTAL-CCDZ201201029.htm -
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