高氯酸盐在硝酸盐还原条件下的厌氧生物降解

王蕊; 刘菲; 秦莉红; 陈鸿汉

doi:10.3799/dqkx.2012.034

高氯酸盐在硝酸盐还原条件下的厌氧生物降解

doi: 10.3799/dqkx.2012.034

中国地质大学水资源与环境学院, 北京 100083

基金项目:

水体污染控制与治理科技重大专项项目 2009ZX07424-002

详细信息

作者简介:
王蕊(1984-), 女, 博士研究生, 主要研究方向为地下水污染与控制.E-mail: wangruijoy@126.com

中图分类号: X52
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- 被引次数: 0
出版历程
- 收稿日期: 2010-09-20
- 刊出日期: 2012-03-15

Anaerobic Biological Reduction of Perchlorate in the Presence of Nitrate

Faculty of Water Resources and Environment, China University of Geosciences, Beijing 100083, China

摘要

摘要: 利用厌氧微生物降解法, 以醋酸根为碳源, 考察了高氯酸盐在不同浓度的硝酸盐还原环境下的生物降解能力, 分析硝酸盐对高氯酸盐生物降解的影响.实验结果表明, 10mg/L的高氯酸盐在不同浓度硝酸盐环境下均可被富集培养物降解.20mg/L硝酸盐还原环境中, 高氯酸盐的降解未受到抑制; 在碳源充足的条件下, 100mg/L、200mg/L及500mg/L的硝酸盐环境中, 高氯酸盐降解出现滞后期, 分别为7d、13d和38d.反应初期, 高氯酸盐降解滞后是由于硝酸盐为优先级别较高的电子受体, 更易于被微生物利用.随着硝酸盐快速降解和亚硝酸盐的累积, 高氯酸盐降解停滞可能是由于电子竞争和较高浓度的亚硝酸盐对高氯酸盐降解菌酶活性产生毒性抑制这两方面共同作用的结果.
- 高氯酸盐 /
- 硝酸盐 /
- 降解 /
- 污染控制 /
- 环境工程
Abstract: Anaerobic biological reduction of perchlorate in the presence of nitrate using is studied acetate as carbon source. In addition, effects of different nitrate concentrations on perchlorate reduction are researched. The results show that 10mg/L of perchlorate can be removed by the enriched microorganisms in different nitrate-reducing conditions. Evidence is also presented demonstrating that perchlorate reduction is not inhibited under 20mg/L nitrate-reducing condition. However, under 100mg/L, 200mg/L and 500mg/L nitrate-reducing conditions, perchlorate removal is observed with longer lag periods of 7d, 13d and 38d respectively. At the initial period of reactions, nitrate, as a more favorable electron acceptor, is more easily used by the microbes. With the rapid reduction of nitrate and nitrite accumulation, it is reasonable to propose that the electronic competition and the toxic effect of a certain level of nitrite, particularly in large quantity, on the activity of perchlorate-reducing bacteria, may temporarily delay perchlorate biological reduction.
- perhclorate /
- nitrate /
- degradation /
- pollution control /
- environmental engineering

HTML全文

图 1 高氯酸盐降解菌的驯化结果

Fig. 1. Acclimation results of perchlorate reduction microorganisms

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图 2 硝酸盐还原环境中NO₃^-的变化情况

Fig. 2. Changes in nitrate concentration in the presence of nitrate conditions

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图 3 硝酸盐还原环境中高氯酸盐的浓度变化情况

Fig. 3. Changes in perchlorate concentration in two control sets and active sets in the absence and the presence of nitrate

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图 4 (a) 100 mg/L、(b)200 mg/L、(c)500 mg/L硝酸盐环境中ClO₄^-、NO₃^-及NO₂^-变化

Fig. 4. Changes in perchlorate and nitrate/nitrite concentration in the active medium in the presence of 100 mg/L nitrate (a)、200 mg/L nitrate (b) and 500 mg/L nitrate (c)

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图 5 (a) 20 mg/L、(b)100 mg/L硝酸盐环境中的Cl^-平衡

Fig. 5. Chloride balance in 20 mg/L (a) and 100 mg/L (b) nitrate-reducing conditions

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参考文献(29)

[1]	Bardiya, N., Bae, J.H., Nusslein, K.R., et al., 2005. Bioremediation potential of a perchlorate-enriched sewage sludge consortium. Chemosphere, 58(1): 83-90. doi: 10.1016/j.chemosphere.2004.09.001
[2]	Cai, Y.Q., Shi, Y.L., Zhang, P., et al., 2006. Perchlorate related environmental problems. Progress in Chemistry, 18(11): 1554-1564 (in Chinese with English abstract). http://en.cnki.com.cn/Article_en/CJFDTOTAL-HXJZ200611017.htm
[3]	Dasgupta, P.K., Martinelango, P.K., Jachson, W.A., et al., 2005. The origin of naturally occurring perchlorate: the role of atmospheric processes. Environ. Sci. Technol. , 39(6): 1569-1575. doi: 10.1021/es048612x
[4]	Dudley, M., Salamone, A., Nerenberg, R., 2008. Kinetics of a chlorate-accumulating, perchlorate-reducing bacterium. Water Res. , 42(10-11): 2403-2410. doi: 10.1016/ j.watres.2008.01.009
[5]	Farhan, Y.H., Hatzinger, P.B., 2009. Modeling the biodegradation kinetics of perchlorate in the presence of oxygen and nitrate as competing electron acceptors. Biorem. J. , 13(2): 2, 65-78. doi: 10.1080/10889860902902016
[6]	Furdui, V.I., Tomassini, F., 2010. Trends and sources of perchlorate in Arctic snow. Environ. Sci. Technol. , 44(2): 588-592. doi: 10.1021/es902243b
[7]	Gal, H., Ronen, Z., Weisbrod, N., et al., 2008. Perchlorate biodegradation in contaminated soils and the deep unsaturated zone. Soil Biol. Biochem. , 40(7): 1751-1757. doi: 10.1016/j.soilbil.2008.02.015
[8]	Jackson, W.A., Bohlke, J.K., Gu, B.H., et al., 2010. Isotopic composition and origin of indigenous natural perchlorate and co-occurring nitrate in the southwestern United States. Environ. Sci. Technol. , 44(13): 4869-4876. doi: 10.1021/es903802j
[9]	Jin, Z.F., Li, W.T., Pan, Z.Y., et al., 2006. Methods for nitrate removal from underground water. Technology of Water Treatment, 32(8): 34-37 (in Chinese with English abstract). http://www.cqvip.com/Main/Detail.aspx?id=22497484
[10]	Ju, X.M., Sierra-Alvarez, R., Field, J.A., et al., 2008. Microbial perchlorate reduction with elemental sulfur and other inorganic electron donors. Chemosphere, 71(1): 114-122. doi: 10.1016/j.chemosphere.2007.09.045
[11]	Lee, J.W., Lee, K.H., Park, K.Y., et al., 2010. Hydrogenotrophic denitrification in a packed bed reactor: effects of hydrogen-to-water flow rate ratio. Bioresour. Technol. , 101(11): 3940-3946. doi: 10.1016/j.biortech.2010.01.022
[12]	Nerenberg, R., Kawagoshi, Y., Rittnann, B.E., 2006. Kinetics of a hydrogen-oxidizing, perchorate-reducing bacterium. Water. Res. , 40(17): 3290-3296. doi: 10.1016/j.watres.2006.06.035
[13]	Nozawa-Inoue, M., Scow, K.M., Rolston, D.E., 2005. Reduction of perchlorate and nitrate by microbial communities in vadose soil. Appl. Environ. Microbiol. , 71(7): 3928-3934. doi: 10.1128/AEM.71.7.3928-3934.2005
[14]	Qian, H.J., Xi, S.L., He, P., et al., 2009. Biological reduction of perchlorate and optimization. Environmental Science, 30(5): 1402-1407 (in Chinese with English abstract). http://europepmc.org/abstract/MED/19558109
[15]	Sahu, A.K., Conneely, T., Nusslein, K.R., et al., 2009. Biological perchlorate reduction in packed bed reactors using elemental sulfur. Environ. Sci. Technol. , 43(12): 4466-4471. doi: 10.1021/es900563f
[16]	Shrout, J.D., Parkin, G.F., 2006. Influence of electron donor, oxygen, and redox potential on bacterial perchlorate degradation. Water Res. , 40(6): 1191-1199. doi: 10.1016/j.watres.2006.01.035
[17]	Son, A., Lee, J., Chiu, P.C., et al., 2006. Microbial reduction of perchlorate with zero-valent iron. Water. Res. , 40(10): 2027-2032. doi: 10.1016/j.watres.2006.03.027
[18]	Srinivasan, A., Viraraghavan, T., 2009. Perchlorate: health effects and technologies for its removal from water resources. Int. J. Environ. Res. Public Health, 6(4): 1418-1442. doi: 10.3390/ijerph6041418
[19]	Tan, K., Anderson, T.A., Jackson, W.A., 2004. Degradation kinetics of perchlorate in sediments and soils. Water, Air, Soil Pollut. , 151(1-4): 245-259. doi: 10.1023/B:WATE.0000009904.23410.89
[20]	Wang, D.M., Shah, S.I., Chen, J.G., et al., 2008. Catalytic reduction of perchlorate by H₂ gas in dilute aqueous solutions. Sep. Purif. Technol. , 60(1): 14-21. doi: 10.1016/j.seppur.2007.07.039
[21]	Wang, H.C., Sun, C., 2009. Inorganic and analytical chemistry experiment. Chemical Industry Publishing House, Beijing, 1-134 (in Chinese).
[22]	Wu, D.L., He, P., Xu, X.H., et al., 2008. The effect of various reaction parameters on bioremediation of perchlorate-contamiated water. J. Hazard. Mater. , 150(2): 419-423. doi: 10.1016/j.jhazmat.2007.04.124
[23]	Xu, J., Trimble, J.J., Steinberg, L., et al., 2004. Chlorate and nitrate reduction pathways are separately induced in the perchlorate-respiring bacterium Dechlorosoma sp. KJ and chlorate-respiring bacterium Pseudomonas sp. PDA. Water Res. , 38(3): 673-680. doi: 10.1016/j.watres.20083.10.017
[24]	Yu, X.Y., Amrhein, C., Deshusses, M.A., et al., 2007. Perchlorate reduction by autotrophic bacteria attached to zero-valent iron in a flow-through reactor. Environ. Sci. Technol. , 41(3): 990-997. doi: 10.1021/es061959a
[25]	Ziv-EI, M.C., Rittmann, B.E., 2009. Systematic evaluation of nitrate and perchlorate bioreduction kinetics in groundwater using a hydrogen-based membrane biofilm reactor. Water Res. , 43(1): 173-181. doi: 10.1016/j.watres.2008.09.035
[26]	蔡亚岐, 史亚利, 张萍, 等, 2006. 高氯酸盐的环境污染问题. 化学进展, 18(11): 1554-1564. doi: 10.3321/j.issn:1005-281X.2006.11.018
[27]	金赞芳, 李文腾, 潘志彦, 等, 2006. 地下水硝酸盐去除方法. 水处理技术, 32(8): 34-37. doi: 10.3969/j.issn.1000-3770.2006.08.009
[28]	钱慧静, 奚胜兰, 何平, 等, 2009. 生物法降解高氯酸盐及其优化研究. 环境科学, 30(5): 1402-1407. doi: 10.3321/j.issn:0250-3301.2009.05.025
[29]	王和才, 孙成, 2009. 无机及分析化学实验. 北京: 化学工业出版社.