东海内陆架泥质区沉积物古菌群落垂向分布特征

张林宝; 李铁刚; 党宏月; 萨仁高娃; 张伟

doi:10.3799/dqkx.2010.025

东海内陆架泥质区沉积物古菌群落垂向分布特征

doi: 10.3799/dqkx.2010.025

张林宝^{1, 2},
李铁刚^1, ,,
党宏月³,
萨仁高娃¹,
张伟^{1, 2}

1.
中国科学院海洋研究所海洋地质与环境重点实验室, 山东青岛 266071
2.
中国科学院研究生院, 北京 100049
3.
中国石油大学(华东)生物工程与技术中心, 山东青岛 266555

基金项目:

中国科学院知识创新重要方向项目 Nos.KZCX2-YW-211-03

中国科学院知识创新重要方向项目 KZCX3-SW-233

详细信息

作者简介:
张林宝(1984-), 女, 硕士研究生, 主要从事地质微生物学研究

通讯作者:
李铁刚, E-mail: tgli@ms.qdio.ac.cn

中图分类号: P736;Q89
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- 被引次数: 0
出版历程
- 收稿日期: 2008-12-23
- 刊出日期: 2010-03-01

Vertical Distribution of Archaea in Mud Wedge Sediments Located at Inner Continental Shelf of the East China Sea

1.
Key Laboratory of Marine Geology and Environment, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
2.
Graduate University of Chinese Academy of Sciences, Beijing 100049, China
3.
Center for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao 266555, China

摘要

摘要: 为研究东海内陆架闽浙沿岸泥质区不同深度沉积物中古菌群落垂向分布特征, 利用古菌16S rDNA基因文库得到473个有效克隆、50个OTUs(Operational Taxonomic Units).16S rDNA序列系统进化和统计分析发现古菌分别归属于泉古生菌(Crenarchaeota)和广古生菌(Euryarchaeota), 其中以Miscellaneous Crenarchaeotic Group(MCG)为主, 仅含少量的Marine Benthic Group B(MBG-B)、South African Gold Mine Euryarchaeotic Group(SAGMEG)、Anaerobic Methanotrophs 3(ANME-3)、Marine Crenarchaeotic Group I(MG-I)和Marine Benthic Group D(MBG-D).该泥质区沉积物可能存在由ANME-3催化的甲烷厌氧氧化作用, 同源序列分析表明其古菌群落分布与周边环境有较大联系.UniFrac与沉积物环境因子分析表明该泥质区古菌群落垂向分布与沉积物有机质含量和粒度变化密切相关.
- 内陆架 /
- 东海 /
- 泥质区 /
- 古菌 /
- 垂向分布 /
- 生物多样性
Abstract: Archaeal 16S rDNA clone libraries were constructed from sediments of different depths to elucidate the distribution and diversity of archaea in the Fujian and Zhejiang coastal mud wedge of the inner continental shelf of the East China Sea. We obtained 473 useable clones and 50 different OTUs (Operational Taxonomic Units). Phylogenetic and statistic analysis of clone libraries show that: archaeal 16S rDNA sequences are within phylums of Crenarchaeota and Euryarchaeota, respectively. The majority of archaeal phylotypes are Miscellaneous Crenarchaeotic Group (MCG), whereas few sequences fall into Marine Benthic Group B (MBG-B), South African Gold Mine Euryarchaeotic Group (SAGMEG), Anaerobic Methanotrophs 3 (ANME-3), Marine Crenarchaeotic Group I (MG-I) and Marine Benthic Group D (MBG-D). There might be anaerobic oxidation of methane carried out by group ANME-3 in the mud wedge. Homologous sequence analyses show that the distribution of archaea in the mud wedge is related to the surrounding environment. UniFrac analysis and sediment environmental parameters indicate that the vertical distribution of archaea closely coincides with the variation of sediment total organic carbon content and grain size.
- inner continental shelf /
- the East China Sea /
- mud wedge /
- archaeal /
- vertical distribution /
- biodiversity

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图 1 东海内陆架泥质区MD06-3040孔采样站位

Fig. 1. Sample site of core MD06-3040 retrieved from the mud wedge in the inner continental shelf of the East China Sea

下载: 全尺寸图片幻灯片

图 2 古菌16S rDNA克隆文库稀释曲线

Fig. 2. Rarefaction curves of archaeal 16S rDNA clones

下载: 全尺寸图片幻灯片

图 3 古菌16S rDNA Jackknife Cluster分析

Fig. 3. Analysis of archaeal 16S rDNA Jackknife Cluster

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图 4 东海内陆架泥质区古菌16S rDNA序列系统发育树

置信度值大于70%的标在相应分支的节点上(经过100次计算)

Fig. 4. Phylogenetic tree of archaeal 16S rDNA sequences recovered from the East China Sea mud wedge

下载: 全尺寸图片幻灯片

表 1 古菌16S rDNA克隆文库分析

Table 1. Analysis of the archaeal 16S rDNA clone libraries

文库	总克隆数	RFLP带型数	OTUs数	覆盖率^*(%)	香农-威纳指数(H′)	辛普森指数(D)	均匀度(J′)
ECS1	101	33	18	92.1	3.4	8.1	0.8
ECS2	98	32	19	91.9	3.4	8.6	0.8
ECS3	98	32	17	90.8	2.9	5.1	0.7
ECS4	94	29	18	94.7	3.4	7.9	0.8
ECS5	87	38	26	86.3	3.9	10.3	0.8

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表 2 古菌16S rDNA文库克隆系统发育归属关系

Table 2. Phylogenetic affiliations of archaeal 16S rDNA clone libraries

系统发育类群	克隆数
系统发育类群	ECS1	ECS2	ECS3	ECS4	ECS5
Euryarchaeota
SAGMEG	13	4	3	5	3
MBG-D	ND ^*	ND	1	ND	1
ANME-3	ND	2	4	ND	3
Crenarchaeota
MCG	82	84	78	81	76
MG-I	ND	1	ND	1	4
MBG-B	6	7	12	7	ND
总计	101	98	98	94	87
注：ND^*，未探测.

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表 3 东海内陆架泥质区沉积物环境因子

Table 3. Environmental parameters of mud wedge sediments in the inner continental shelf of the East China Sea

深度(m)	总有机碳TOC(%)	中值粒径	平均粒径	砂(%)	粉砂(%)	粘土(%)
10.5	0.56	7.2	7.4	0	64.7	35.3
12	0.58	7.5	7.7	0	59.8	40.2
15	0.59	7.4	7.6	0	59.8	40.2
18	0.57	6.8	7.2	0	67.7	32.3
19.4	0.34	4.3	4.9	47.7	37.4	14.9

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