A Preliminary Phylogenetic Analysis of ITS nrDNA Sequences in Water-lilies
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摘要: 睡莲类植物是目前植物分子系统学与进化研究的一个重要类群.核基因组的ITS区是核核糖体(nrDNA) 转录单位的一部分.测定和分析了5个属的7种睡莲类植物的核核糖体ITS序列, 并与GenBank中提取的相关序列进行了组合分析, 初步获得了睡莲类植物的2个ITS系统树, 结果也支持现有分子系统学研究中有关金鱼藻处于较原始位置的观点.Abstract: Water-lily plants are key group on study of the flowering plant phylogeny. The internal transcribed spacers (ITS) and the 5.8S coding region of nuclear ribosomal DNA (nrDNA) of seven taxa were sequenced to study molecular phylogeny and evolution of Nymphaeales sensu lato. The ITS phylogenetic trees were constructed based on the sequences and a combined data set with sequences from GenBank. The results obtained from ITS sequences also supported that the genus Ceratophyllum could be considered the ancestral group in the order, as the results of most molecular systematic studies.
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图 1 用简约法和邻接法构建的ITS系统树
数字表示各分支自展数据支持率; *表示外类群, 分类群缩写见表 1, 左为MP, 右为NJ
Fig. 1. ITS phylogenetic trees using maximum parsimony and neighbor-joining methods
图 2 用简约法构建的ITS系统树(组合数据集)
数字表示各分支自展数据支持率; *表示外类群; 分类群缩写见表 1; 右边斜体为属名
Fig. 2. ITS phylogenetic trees using maximum parsimony (combined data set)
表 1 实验材料的种名、缩写及来源
Table 1. Species, abbreviation and source
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