通辽科尔沁地区土壤微生物群落结构和功能及其影响因素

肖玉娜; 钟信林; 王北辰; 杨渐; 蒋宏忱

doi:10.3799/dqkx.2019.067

Volume 45 Issue 3

Mar. 2020

Turn off MathJax

Article Contents

Article Navigation > Earth Science > 2020 > 45(3): 1071-1081

Xiao Yuna, Zhong Xinlin, Wang Beichen, Yang Jian, Jiang Hongchen, 2020. Microbial Community Structure and Function and Their Influencing Factors in the Soil of Horqin Area of Tongliao City, Inner Mongolia. Earth Science, 45(3): 1071-1081. doi: 10.3799/dqkx.2019.067

Citation:

Xiao Yuna, Zhong Xinlin, Wang Beichen, Yang Jian, Jiang Hongchen, 2020. Microbial Community Structure and Function and Their Influencing Factors in the Soil of Horqin Area of Tongliao City, Inner Mongolia. Earth Science, 45(3): 1071-1081. doi: 10.3799/dqkx.2019.067

Citation:

Xiao Yuna, Zhong Xinlin, Wang Beichen, Yang Jian, Jiang Hongchen, 2020. Microbial Community Structure and Function and Their Influencing Factors in the Soil of Horqin Area of Tongliao City, Inner Mongolia. Earth Science, 45(3): 1071-1081. doi: 10.3799/dqkx.2019.067

PDF( 1808 KB)

Microbial Community Structure and Function and Their Influencing Factors in the Soil of Horqin Area of Tongliao City, Inner Mongolia

doi: 10.3799/dqkx.2019.067

1.
School of Environmental Studies, China University of Geosciences, Wuhan 430074, China
2.
State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan 430074, China

Received Date: 2019-02-20
Publish Date: 2020-03-15

Abstract

Abstract

As a traditional industry in the Horqin area of Tongliao City,agriculture plays a pivotal role in the development of economy. Understanding the structure and function of soil microbial communities and their influencing factors is of great significance for crop planting and remediation of contaminated land. In this study,microbial community and function composition were investigated in a total of 71 soil samples collected from the Horqin area of Tongliao City with an integrated approach including 16S rRNA gene-based Illumina Miseq high-throughput sequencing technology and FAPROTAX.1.1 functional prediction platform,followed by statistical analysis with the use of R software package. The results show that the microbial communities in the studied soils were mainly composed of Proteobacteria (19.77%),Thaumarchaeota (17.85%),Acidobacteria (17.14%),Actinobacteria (15.58%). The functional prediction indicates that a large portion of the microbial communities in the studied soils were involved in nitrogen cycling,among which aerobic ammonia oxidation function predominated. Variation partition analysis (VPA) indicates that environmental factors accounted much more for the shaping of microbial community structure in interpretable range than spatial factors. Mantel test shows that the microbial communities in the studied soils were significantly (p < 0.05) correlated with soil pH,electrical conductivity (EC),total nitrogen (TN),ratio of carbon to nitrogen,and concentration of Mg,Na and Sr elements. It is concluded that pH,EC and Sr element concentrations were important environmental factors affecting the microbial community composition,function and diversity in the soils of the Horqin area of Tongliao City.
- soil,
- microbial community,
- population composition,
- function,
- environmental response,
- ecology

FullText(HTML)

References(53)

References

Borcard D., Legendre P., Avois-Jacquet C., et al.2004. Dissecting the Spatial Structure of Ecological Data at Multiple Scales. Ecology, 85(7):1826-1832.https://doi.org/10.1890/03-3111 doi: 10.1890-03-3111/

Brockett B. F. T., Prescott C. E., Grayston S. J.. 2012. Soil Moisture is the Major Factor Influencing Microbial Community Structure and Enzyme Activities across Seven Biogeoclimatic Zones in Western Canada. Soil Biology and Biochemistry, 44(1): 9-20. https://doi.org/10.1016/j.soilbio.2011.09.003 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=d0532e75a979fc1cf813ed8009af109f

Chaer G., Fernandes M., Myrold D., et al. 2009. Comparative Resistance and Resilience of Soil Microbial Communities and Enzyme Activities in Adjacent Native Forest and Agricultural Soils. Microbial Ecology, 58(2): 414-424. https://doi.org/10.1007/s00248-009-9508-x http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=87fdf61b563f4f8a0584733c4948f18d

Chen M.L., Zeng Q.C., Huang Y.M., et al. 2018. Effects of the Farmland-to-Forest/Grassland Conversion Program on the Soil Bacterial Community in the Loess Hilly Region. Environmental Science, 39(4): 1824-1832 (in Chinese with English abstract). http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=hjkx201804044

Chu H. Y., Fierer N., Lauber C. L., et al. 2010. Soil Bacterial Diversity in the Arctic is not Fundamentally Different from that Found in other Biomes. Environmental Microbiology, 12(11): 2998-3006. https://doi.org/10.1111/j.1462-2920.2010.02277.x

Chu H. Y., Lin X. G., Fujii T., et al. 2007. Soil Microbial Biomass, Dehydrogenase Activity, Bacterial Community Structure in Response to Long-Term Fertilizer Management.Soil Biology and Biochemistry, 39(11): 2971-2976. https://doi.org/10.1016/j.soilbio.2007.05.031

Degrune F., Dufrêne M., Colinet G., et al. 2015. A Novel Subphylum Method Discriminates Better the Impact of Crop Management on Soil Microbial Community. Agronomy for Sustainable Development, 35(3): 1157-1166. https://doi.org/10.1007/s13593-015-0291-4

Ding Z.L., Wu J.P., You A.Q., et al.2017.Effects of Heavy Metals on Soil Microbial Community Structure and Diversity in the Rice (Oryza Sativa L. subsp. Japonica, Food Crops Institute of Jiangsu Academy of Agricultural Sciences) Rhizosphere. Soil Science & Plant Nutrition, 63(1):75-83. https://doi.org/10.1080 /00380768.2016.1247385

Edgar R. C.. 2013. UPARSE: Highly Accurate OTU Sequences from Microbial Amplicon Reads. Nature Methods, 10(10): 996-998. https://doi.org/10.1038/nmeth.2604 http://d.old.wanfangdata.com.cn/NSTLQK/NSTL_QKJJ0231145773/

Fierer N., Bradford M. A., Jackson R. B.. 2007. Toward an Ecological Classification of Soil Bacteria. Ecology, 88(6): 1354-1364. https://doi.org/10.1890/05-1839 doi: 10.1890-05-1839/

Garbeva P., Postma J., van Veen J. A., et al. 2006. Effect of Above-Ground Plant Species on Soil Microbial Community Structure and Its Impact on Suppression of Rhizoctonia Solani AG3. Environmental Microbiology, 8(2): 233-246. https://doi.org/10.1111/j.1462-2920.2005.00888.x http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=4a758f5903563a1eab7cbe29e78d52bb

Guan X. Y., Wang J. F., Zhao H., et al. 2013. Soil Bacterial Communities Shaped by Geochemical Factors and Land Use in a Less-Explored Area, Tibetan Plateau. BMC Genomics, 14(1): 820. https://doi.org/10.1186/1471-2164-14-820

Isobe K., Koba K., Suwa Y., et al. 2012. High Abundance of Ammonia-Oxidizing Archaea in Acidified Subtropical Forest Soils in Southern China after Long-Term N Deposition. FEMS Microbiology Eco-logy, 80(1): 193-203. https://doi.org/10.1111/j.1574-6941.2011.01294.x http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=7aca8b3c486924085ab2c033448b4459

Jiang H.C., Hang L.Q., Deng Y., et al.2014.Latitudinal Distribution of Ammonia-Oxidizing Bacteria and Archaea in the Agricultural Soils of Eastern China. Applied and Environmental Microbiology, 80(18):5593-5602.https://doi.org/10.1128/aem.01617-14 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=4c3c209e7e343f50cb08195de03794a0

Kennedy A. C., Smith K. L.. 1995. Soil Microbial Diversity and the Sustainability of Agricultural Soils. Plant and Soil, 170(1): 75-86. https://doi.org/10.1007/bf02183056 http://d.old.wanfangdata.com.cn/OAPaper/oai_doaj-articles_4241b49ddefcd4e7b3340e0fbe6d9562

Khan S., El-Latif Hesham A., Qiao M., et al. 2010. Effects of Cd and Pb on Soil Microbial Community Structure and Activities. Environmental Science and Pollution Research, 17(2): 288-296. https://doi.org/10.1007/s11356-009-0134-4 doi: 10.1016-j.ecoleng.2011.07.002/

Lauber C.L., Hamady M., Knight R., et al.2009.Pyrosequencing-Based Assessment of Soil pH as a Predictor of Soil Bacterial Community Structure at the Continental Scale. Applied Environmental Microbiology, 75(15):5111-5120.https://doi.org/10.1128/aem.00335-09 doi: 10.1128-AEM.00335-09/

Lauber C. L., Strickland M. S., Bradford M. A., et al. 2008. The Influence of Soil Properties on the Structure of Bacterial and Fungal Communities across Land-Use Types. Soil Biology and Biochemistry, 40(9): 2407-2415. https://doi.org/10.1016/j.soilbio.2008.05.021 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=59bd9fb69b2ee0a0f7d22a2f6e0d75d6

Li N., Shao T. Y., Zhu T. S., et al. 2018. Vegetation Succession Influences Soil Carbon Sequestration in Coastal Alkali-Saline Soils in Southeast China. Scientific Reports, 8(1):9728-9739.https://doi.org/10.1038/s41598-018-28054-0

Liu Y., Huang Y.M., Zeng Q.C.. 2016. Soil Bacterial Communities under Different Vegetation Types in the Loess Plateau. Environmental Science, 37(10): 3931-3938 (in Chinese with English abstract). http://d.old.wanfangdata.com.cn/Periodical/hjkx201610035

Louca S., Parfrey L. W., Doebeli M.. 2016. Decoupling Function and Taxonomy in the Global Ocean Microbiome. Science, 353(6305): 1272-1277. https://doi.org/10.1126/science.aaf4507 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=2f05c6bb2e6feefcc778ff870b43476a

Lynch M. D. J., Neufeld J. D.. 2015. Ecology and Exploration of the Rare Biosphere. Nature Reviews Microbiology, 13(4): 217-229. https://doi.org/10.1038/nrmicro3400

Ma Z.Z., Qiao S.S., Cao M.W., et al. 2018.Environmental Selection and Dispersal Limitation Drive the Assemblage of Bacterial Community in Temperate Forest Soils. Chinese Journal of Applied Ecology, 29(4): 1179-1189 (in Chinese with English abstract). http://d.old.wanfangdata.com.cn/Periodical/yystxb201804019

Martiny J. B., Bohannan B.J., Brown J.H., et al. 2006.Microbial Biogeography: Putting Microorganisms on the Map. Nature Reviews. Microbiology, 4(2): 102-112. https://doi.org/10.1038/nrmicro1341 http://d.old.wanfangdata.com.cn/OAPaper/oai_doaj-articles_bf3d74a0fb5c4b90c8e46eb4b560fc0a

Marusenko Y., Bates S.T., Anderson I., et al. 2013. Ammonia-Oxidizing Archaea and Bacteria are Structured by Geography in Biological Soil Crusts across North American Arid Lands. Ecological Processes, 2(1):9-19. https://doi.org/10.1186/2192-1709-2-9

Pereira C.S., Lopes I., Abrantes I., et al.2019.Salinization Effects on Coastal Ecosystems: A Terrestrial Model Ecosystem Approach. Philosophical Transactions of the Royal Society B:Biological Sciences, 374(1764):20180251. https://doi.org/10.1098/rstb.2018.0251

Qi L., Li Y.L., Zhao W., et al. 2018. Effect of Avena Sativa L. on Soil Enzyme Activity and Microbe Functional Diversity under Strontium Pollution. Acta Ecologica Sinica, 38(13): 4888-4896 (in Chinese with English abstract). http://d.old.wanfangdata.com.cn/OAPaper/oai_doaj-articles_eff7ec6f64ead86411810ab6de60daa2

Shen C. C., Xiong J. B., Zhang H. Y., et al. 2013. Soil pH Drives the Spatial Distribution of Bacterial Communities along Elevation on Changbai Mountain. Soil Biology and Biochemistry, 57: 204-211. https://doi.org/10.1016/j.soilbio.2012.07.013

Stopnisek N., Bodenhausen N., Frey B., et al.2014.Genus-wide Acid Tolerance Accounts for the Biogeographical Distribution of Soil Burkholderia Populations. Environmental Microbiology, 16(6):1503-1512.https://doi.org/10.1111/1462-2920.12211 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=3320fa16cc5b60c79d328e3e3ae7b1e3

Tang J., Ding X., Wang L.M., et al. 2012. Effects of Wetland Degradation on Bacterial Community in the Zoige Wetland of Qinghai-Tibetan Plateau (China). World Journal of Microbiology and Biotechnology, 28(2): 649-657. https://doi.org/10.1007/s11274-011-0858-4 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=d008553d9eb8ae19f365295109aed9d6

Tang Y., Liu Y.C., Yang J., et al. 2018. Gene Diversity Involved in Kalvin Pathway of Carbon Fixation and Its Response to Environmental Variables in Surface Sediments of the Northern Qinghai-Tibetan Plateau Lakes. Earth Science, 43(S1): 19-30 (in Chinese with English abstract). http://d.old.wanfangdata.com.cn/Periodical/dqkx2018z1003

Uddin M., Chen J. W., Qiao X. L., et al. 2019.Bacterial Community Variations in Paddy Soils Induced by Application of Veterinary Antibiotics in Plant-Soil Systems. Ecotoxicology and Environmental Safety, 167: 44-53. https://doi.org/10.1016/j.ecoenv.2018.09.101 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=ffab20446ac91d45e4f1d1079c8a3aca

Wang X., Chen C., Wang J.L.. 2017. Phytoremediation of Strontium Contaminated Soil by Sorghum Bi-Color (L.) Moench and Soil Microbial Community-Level Physiological Profiles (CLPPs).Environmental Science and Pollution Research, 24(8): 7668-7678. https://doi.org/10.1007/s11356-017-8432-8

Wei F.S., Yang G.Z., Jiang D.Z., et al. 1991. Basic Statistics and Characteristics of Soil Element Background Value in China. Environmental Monitoring in China, 7(1): 1-6 (in Chinese).

Xu Y., Wang H.M., Xiang X., et al.2019.Vertical Variation of Nitrogen Fixers and Ammonia Oxidizers along a Sediment Profile in the Dajiuhu Peatland, Central China.Journal of Earth Science, 30(2):397-406. https://doi.org/10.1007/s12583-018-0982-2 http://d.old.wanfangdata.com.cn/Periodical/dqkx-e201902015

Zhang L.M., He J.Z.. 2012. A Novel Archaeal Phylum: Thaumarchaeota: A Review. Acta Microbiologica Sinica, 52(4): 411-421 (in Chinese with English abstract). http://d.old.wanfangdata.com.cn/OAPaper/oai_pubmedcentral.nih.gov_2292581

Zhang Y.M., Wu G., Jiang H.C.. 2018. Research Progress on Microorganisms Driving Carbon Cycle in Hot Spring. Earth Science, 43(S1): 31-41 (in Chinese with English abstract). https://www.sciencedirect.com/science/article/pii/S1006810414600362

Zhang Y. M., Wu G., Jiang H. C., et al. 2018. Abundant and Rare Microbial Biospheres Respond Differently to Environmental and Spatial Factors in Tibetan Hot Springs. Frontiers in Microbiology, 9:2096. https://doi.org/10.3389/fmicb.2018.02096

Zhao A.J., Hu T.X., Chen X.H.. 2009. Multiple-Scale Spatial Analysis of Community Structure in a Mountainous Mixed Evergreen-Deciduous Broad-Leaved Forest, Southwest China. Biodiversity Science, 17(1): 43-50 (in Chinese with English abstract). http://d.old.wanfangdata.com.cn/OAPaper/oai_doaj-articles_8f2e64c39236cf52f1ca1283f30c029e

Zhao J., Zhang R. F., Xue C., et al. 2014. Pyrosequencing Reveals Contrasting Soil Bacterial Diversity and Community Structure of Two Main Winter Wheat Cropping Systems in China. Microbial Ecology, 67(2): 443-453. https://doi.org/10.1007/s00248-013-0322-0 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=36526d24a86bfe57f22dc8b0730abe6b

Zhao M., Yin C.S., Li C.W., et al. 2018. Using Miseq Sequencing to Analyze Seasonal Soil Microbial Community Dynamics in Reclaimed Scirpus Mariqueter Coastal Wetlands. Journal of Shanghai Ocean University, 27(5): 718-727 (in Chinese with English abstract). http://d.old.wanfangdata.com.cn/Periodical/shscdxxb201805009

Zhu L.Y., Ding M.Y., Huang G.N., et al. 2018. Microbial Community Structure and the Correlation between Environmental Factors and Community Structure in Sandy Soil of Tongliao in Inner Mongolia. Science Technology and Engineering, 18(20): 347-352 (in Chinese with English abstract). http://d.old.wanfangdata.com.cn/Periodical/kxjsygc201820052

陈孟立, 曾全超, 黄懿梅, 等. 2018.黄土丘陵区退耕还林还草对土壤细菌群落结构的影响.环境科学, 39(4): 1824-1832. http://d.old.wanfangdata.com.cn/Periodical/hjkx201804044

刘洋, 黄懿梅, 曾全超. 2016.黄土高原不同植被类型下土壤细菌群落特征研究.环境科学, 37(10): 3931-3938. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=hjkx201610035

马转转, 乔沙沙, 曹苗文, 等. 2018.环境选择和扩散限制驱动温带森林土壤细菌群落的构建.应用生态学报, 29(4): 1179-1189. http://d.old.wanfangdata.com.cn/Periodical/yystxb201804019

亓琳, 李艳玲, 赵威, 等. 2018.锶污染下燕麦对土壤酶活性和微生物群落功能多样性的影响.生态学报, 38(13): 4888-4896. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=stxb201813034

唐阳, 刘永超, 杨渐, 等. 2018.青藏高原北部湖泊表层沉积物参与卡尔文循环的固碳基因多样性及其影响因素.地球科学, 43(S1): 19-30. http://d.old.wanfangdata.com.cn/Periodical/dqkx2018z1003

魏复盛, 杨国治, 蒋德珍, 等. 1991.中国土壤元素背景值基本统计量及其特征.中国环境监测, 7(1): 1-6. http://www.cnki.com.cn/Article/CJFDTotal-IAOB199101000.htm

张丽梅, 贺纪正. 2012.一个新的古菌类群:奇古菌门(Thaumarchaeota).微生物学报, 52(4): 411-421. http://www.cnki.com.cn/Article/CJFDTotal-WSXB201204003.htm

张艳敏, 吴耿, 蒋宏忱. 2018.热泉中驱动碳循环的微生物研究进展.地球科学, 43(S1): 31-41. http://d.old.wanfangdata.com.cn/Periodical/dqkx2018z1004

赵安玖, 胡庭兴, 陈小红. 2009.西南山地阔叶混交林群落空间结构的多尺度特征.生物多样性, 17(1): 43-50. http://d.old.wanfangdata.com.cn/Periodical/swdyx200901005

赵萌, 印春生, 厉成伟, 等. 2018. Miseq测序分析围垦后海三棱藨草湿地土壤微生物群落多样性的季节变化.上海海洋大学学报, 27(5): 718-727. http://d.old.wanfangdata.com.cn/Periodical/shscdxxb201805009

朱立月, 丁美月, 黄冠南, 等. 2018.内蒙古通辽市砂质土壤中微生物群落结构及其与环境因子的关系.科学技术与工程, 18(20): 347-352. http://d.old.wanfangdata.com.cn/Periodical/kxjsygc201820052

Relative Articles

Supplements(1)

Supplements

肖玉娜附表.docx

Cited By

Proportional views

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Figures(7) / Tables(1)

Get Citation

PDF

XML

Article views (2492) PDF downloads(52)

Microbial Community Structure and Function and Their Influencing Factors in the Soil of Horqin Area of Tongliao City, Inner Mongolia

doi: 10.3799/dqkx.2019.067

Abstract

References

Supplements

Proportional views

Catalog

通讯作者: 陈斌, bchen63@163.com

Proportional views

Export File

Citation

Format

Content